Time course of StCyP mRNA accumulation in potato
tubers in response to wounding and fungal infection. (A) The
histogram represents the average of at least three independent
measurements performed with different RNA preparations.
Dashed bars: wounded plus PDA-treated tubers, black bars:
wounded plus F. eumartii. Error bars represent the S.D.
Shared lower case letters (a and b) among bars indicate
statistically indistinguishable values at PB0.05. The amount
of hybridizing RNA measured in non-wound-non-inoculated
tubers has been taken as the 100% value. (B) Autoradiograms
from a single Northern blot experiment. The membrane was
hybridized with the StCyP cDNA probe and exposed for 1
week at 80°C (upper panel). Next, the membrane was
washed and rehybridized with the rDNA probe to test equal
loading of total RNA:lane (lower panel). Lane 0, non-woundnon-
inoculated tubers. W, wounded plus PDA-treated tubers;
WF, wounded plus F. eumartii.