At time of zero minutes and after 30 minutes and 2 hours, subculture an inoculum
(approximately 0.01ml) or a loop full onto Soyabean Casein Digest Agar (M290) using streak plate technique. If desired SCDA
may be also enriched with 5% v/v sheep blood depending on intended organisms to be isolated. Incubate plates at 35±2ºC
for 18-24 hours.