Pitch canker disease, caused by Fusarium circinatum (F. circinatum) Nirenberg and O’Donnell, is an emergent disease that is threatening Pinus trees in Europe. Since it was first recognized in Southeastern USA [1], the disease has been reported in other parts of the world [2]. In many of these reports, the disease appeared first in forest nurseries, like in South Africa [3], Chile [4], Spain [5], Portugal [6] and Brazil [7]. In trees, F. circinatum causes branch dieback and resinous cankers that progressively girdle the wood causing branch death. Canker infection of multiple branches of the main stem can cause extensive dieback in the tree crown and tree mortality.