Results: In the present study, we demonstrated that application of GA3 to ‘Kyoho’ grapevine inflorescences at
pre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion. In an attempt to
obtain a deeper understanding of the molecular mechanisms driving these responses to GA3 treatment, we
performed large-scale transcriptome sequencing of grape flowers following GA3 treatment using Illumina sequencing
technology. Global expression profiles of GA3-treated and untreated grape flowers were compared and a large number
of GA3-responsive genes were identified. Gene ontology (GO) term classification and biochemical pathway analyses
indicated that GA3 treatment caused changes in the levels of transcripts involved in cellular processes, reproduction,
hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS).
These findings suggest that GA3-induced morphological alterations may be related to the control of hormone
biosynthesis and signaling, regulation of transcription factors, alteration of secondary metabolites, and the
stability of redox homeostasis.
Conclusions: Taken together, this comprehensive inflorescence transcriptome data set provides novel insight
into the response of grape flowers to GA3 treatment, and also provides possible candidate genes or markers
that could be used to guide future efforts in this field.
Keywords: Grape, Gibberellic acid, Gene expression, Pathways, RNA-seq, Transcriptome
Results: In the present study, we demonstrated that application of GA3 to ‘Kyoho’ grapevine inflorescences atpre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion. In an attempt toobtain a deeper understanding of the molecular mechanisms driving these responses to GA3 treatment, weperformed large-scale transcriptome sequencing of grape flowers following GA3 treatment using Illumina sequencingtechnology. Global expression profiles of GA3-treated and untreated grape flowers were compared and a large numberof GA3-responsive genes were identified. Gene ontology (GO) term classification and biochemical pathway analysesindicated that GA3 treatment caused changes in the levels of transcripts involved in cellular processes, reproduction,hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS).These findings suggest that GA3-induced morphological alterations may be related to the control of hormonebiosynthesis and signaling, regulation of transcription factors, alteration of secondary metabolites, and thestability of redox homeostasis.Conclusions: Taken together, this comprehensive inflorescence transcriptome data set provides novel insightinto the response of grape flowers to GA3 treatment, and also provides possible candidate genes or markersthat could be used to guide future efforts in this field.Keywords: Grape, Gibberellic acid, Gene expression, Pathways, RNA-seq, Transcriptome
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