Most importantly, the number of particles sampled will be potentially orders of magnitude fewer than those of ensemble methods. While this is a necessary sacrifice, as those methods often have difficulty differentiating individual particles from agglomerates, it potentially decreases the precision of the test, and the increasing variancemay result in reduced occurrence of statistically significant results. Additionally, since the sample preparation includes drying, this prevents in situ analysis and may result in a bias towards sampling of certain particles that may be more prevalent in the surface layer after preparation.
Most importantly, the number of particles sampled will be potentially orders of magnitude fewer than those of ensemble methods. While this is a necessary sacrifice, as those methods often have difficulty differentiating individual particles from agglomerates, it potentially decreases the precision of the test, and the increasing variancemay result in reduced occurrence of statistically significant results. Additionally, since the sample preparation includes drying, this prevents in situ analysis and may result in a bias towards sampling of certain particles that may be more prevalent in the surface layer after preparation.
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