The hydrolysis was carried out in a 3L bioreactor with 1L working
volume at 60 C, 500 rpm. Blended domestic FW (180 g dry)
was inoculated with the fungal mash (7.7 g dry). Then, the total
volume of the blend was adjusted to 1L by adding distilled water.
The reaction was conducted at 60 C with 500 rpm mixing speed.
The pH was not controlled as it was in the range of 4.0–4.5 during
the hydrolysis. Hydrolysis samples were taken at several time
intervals and centrifuged at 10,000 rpm for 10 min for the analyses.
At the end of the hydrolysis, the medium was centrifuged at
10,000 rpm for 20 min and then filtered by vacuum filtration using
Whatman No. 1 filter paper. The filtrate was kept at 20 C and
was subsequently used as the sole fermentation feedstock in the
subsequent ethanol fermentation. All experiments were carried
out in duplicate.