A 10 g portion of the samples were aseptically transferred into
sterile stomacher bag and homogenized in 90 ml sterile saline
(0.85% NaCl) for 60 s in a stomacher (Bagmixer 400W, Interscience,
St. Nom, France). Tenfold serial dilution of fish homogenates were
used for enumeration of bacteria. Total mesophilic and total psychrotrophic
bacteria were enumerated using nutrient agar, incubated
at 35 C for 24 h, and at 7 C for 10 days, respectively. The
results were expressed as log10 cfu/g of the samples.