Table 2
Summary of the 2-step purification approach of 19 recombinant HRP isoenyzmes. The
purification factor (PF) and the recovery of HRP activity in percentage (R%) of the
applied HCIC and AEC flowthrough steps, as well as the combined purification results
are shown. Some isoenzymes did not show any detectable peroxidase activity. In
these cases, no values for PF or R% are available (n/a). The combined PF value is a
product of the PFs of the HCIC and AEC step; in case no values were available for one
purification step (n/a), the combined value only describes the working step.