Banana is a nutritionally as well as economically important plant for which (Basrai variety) an efficient
micro-propagation protocol was developed by using micro-stem cutting, as an explant. The maximum
numbers of plantlets with higher chlorophyll and lower carotenoid contents were observed, which
developed through routinely used tissue culture system (10.0 µM BA; 8.0 µM IAA; 3.0 g/L phytagel) for
organogenesis, permanent immersion system (10.0 µM BA; 1.0 g/L phytagel) for shoot induction and
temporary immersion system (10.0 µM BA; 2.0 g/L phytagel) for shoot multiplication. The developed
plantlets were cultured on 0.5 MS medium with IBA (0.01 mg/L). Rooted plantlets were successfully
transferred to field after initial acclimatization