In both SlCRF3 and SlCRF5, the highest GUS expression was observed in 35 d old plants compared to 15 and 25 d old leaf stages, consistent with RT-PCR analysis. ProSlCRF5 driven GUS expression was also observed in vasculature of cotyledons and leaves in arabidopsis plants transformed with proSlCRF5::GUS cassette (Fig. 2B, j and k). While proSlCRF5::GUS expression was generally the strongest in the vasculature especially in the phloem, its expression could also be observed in other tissues.