Canine granulosa cells were prepared in the same
manner as BGML samples. However, these granulosa
cells were obtained by repeatedly slicing the ovarian
cortex with a scalpel blade at 37 °C, and then the COCs
were suspended in 0.2% of hyaluronidase for 30 to 40
min at 38.5 °C to disperse the granulosa cells.