[21] and Murthy et
al. [22] showing the adverse influence of constant exposure to
TDZ on the multiplication and growth of pea and chickpea.
The recent achievements regarding the adverse effect of TDZ
can be best described by virtue of the capacity of TDZ in
stimulating endogenous cytokinin biosynthesis or altering
cytokinin metabolism [23, 24], and it is necessary to remove
TDZ in subsequent subcultures in order to increase the
multiplication rates or the sturdiness of the shoot. In contrast,
the findings of the current study showed that the continuous
presence of BAP is imperative for shoot multiplication media.
Constant presence of BAP at optimal concentrations was
necessary at different subcultures and there seemed to be a
cumulative gain over subcultures in both the number as well
as the sturdiness of the shoots. These results clearly showed
that TDZ was inferior to BAP in clone Iran 100.