Mature fruit of A. catechu L. were collected from the southern part of Thailand. The pericarp was separated from the fruit, cut into small pieces and dried in a hot air oven at 40 ◦C for 48 h. The phytopathogenic fungus C. gloeosporioides was isolated from diseased tissues of symptomatic mango fruit. The virulence and pathogenicity of the isolates were verified by inoculating mango fruit with 5µL of a spore pathogen suspension. The pathogen was purified via single spore isolation on potato dextrose agar (PDA) and maintained in PDA slants at 10 ◦C (Cloh, 1999).