Plants were harvested 12 days after the start cultivation and placed in a plastic container coated with aluminum foil. Plants were dried to constant weight in an oven at 65 C for 48 h, ground in a blender to a powder, and sieved to uniform consistency. Five grams of the powdered biomass were dissolved in 4 mL of 25 mM sodium acetate at pH 5.5, then brought up to a volume of 100 mL with distilled water (Yu et al., 2014). The resulting solution was incubated at 90 C for 30 min and filtered through Whatmann filter paper no. 1 to remove solids.