In the present study, we explored the relationship between individual differences (i.e., gender and personality traits) and trustworthiness judgments of unfamiliar and emotionally neutral faces. The results suggest that these judgments are affected by the gender of the perceiver, although this effect depends on the valence of the face. Women tend to judge trustworthy-looking faces as significantly more trustworthy than men do. There were no gender differences for judgments of untrustworthy-looking or neutral faces. Moreover, unlike men, women’ trustworthiness judgments are also affected by the gender of the face. Specifically, women judge faces of other women as slightly more trustworthy compared to male faces.