Effect of polymer composition (amine mol-%) and addition sequence on enzymatic microalgal cell wall disruption. The algae concentration = 10 mg/mL,
enzyme loading = 6%
(g enzyme/g total working weight, equivalent to 0.1 g EP/g BDW), polymer loading = 0.2 g/g BDW, pH = 4.8, mixing speed = 120 rpm, and mixing time = 6 h at 30 °C