Blood samples were collected in 2 9 mL Na-heparin tubes (Greiner BioOne GmbH,
Kremsmünster, Austria) and in 2 9 mL, Z serum clot activated tubes (Greiner BioOne GmbH, Kremsmünster,Austria) and placed on ice. Plasma was harvested after
centrifugation at 3000 g for 10 min at 4 1C and serum