Considering the numerous reports of mitotic aberrations in
calli (Mythili et al. 1995; Ezura and Oozawa, 1994;
Fluminhan and Kameya, 1996; Joachimiak et al., 1995; Lee
and Phillips, 1988), especially in tissues incubated in vitro
during long periods of time, we were interested in the
evaluation of this tissue. The chromosome squashes of calli
showed the majority of cells (75 %) undergoing normal
mitosis (Fig. 1c and 1d) and a 25 % of the cells displayed
abnormal mitosis, including changes in chromosome
numbers such as aneuploids with chromosome numbers
lower than 2n = 44, and polyploids (Fig. 2c),
Considering the numerous reports of mitotic aberrations incalli (Mythili et al. 1995; Ezura and Oozawa, 1994;Fluminhan and Kameya, 1996; Joachimiak et al., 1995; Leeand Phillips, 1988), especially in tissues incubated in vitroduring long periods of time, we were interested in theevaluation of this tissue. The chromosome squashes of callishowed the majority of cells (75 %) undergoing normalmitosis (Fig. 1c and 1d) and a 25 % of the cells displayedabnormal mitosis, including changes in chromosomenumbers such as aneuploids with chromosome numberslower than 2n = 44, and polyploids (Fig. 2c),
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