Microbial culture examination
With a 4 mm diameter platinum loop 0.01 ml of the sample was streaked on 5% ovine blood agar plates.
The plates were incubated aerobically at 37C for 24 h.
The resulting growth from the respective plates
of media was purified and identified on the basis of morphology,
colony characteristics and Gram’s reaction (Hokmabad et al., 2011).