In this work we combine the successful NMR approach to polyacid
titration with the pure, biosynthetic hyaluronan from a B. subtilis
engineered to excrete a minimum of bi-products. Further, this
strain is cultivated on a chemically defined minimal media, free of
any peptides (as opposed to streptococcal fermentations) (Widner
et al., 2005). To the authors’ knowledge, no work has previously
been presented using such a pure and well characterised hyaluronan sample.