Indigenous bacteria were isolated from commercial fish sauce
tanks at various fermentation times between 1 and 8 months. A
total of 28 isolates were obtained and identified by 16S rDNA
sequence analysis if they are not reference strains (Table 1). Isolates
identified to be Tetragenococcus and Virgibacillus were cultured
using the media and conditions described above. Other isolates
were grown on tryptic soy agar (TSA) and incubated at 35 C under
aerobic condition for 24 h. Six reference strains, including
T. halophilus ATCC33315, Tetragenococcus muriaticus JCM10006,
Staphylococcus condimenti JCM6074, Staphylococcus carnosus
TISTR833, Staphylococcus piscifermentans JCM6057, and Virgibacillus
halodenitrificans ATCC12304, were also tested. Tetragenococcus and
Staphylococcus were cultured using MRS5 and TSA medium,
respectively. These strains were used for the specificity test.