which originally contained
0.11% (or 7.5 mM) calcium chloride (Abeydeera and Day,
1997) and was further supplemented with 1.1 mg/mL
calcium lactate, whereas BTS does not include calcium
chloride (Pursel and Johnson, 1975). That high calcium
concentration likely favored calcium influx, which is necessary
for the acrosome reaction during incubation. Even
though mTBM does not include sodium bicarbonate, which
is present in BTS, such chemical does not influence the
activation of the adenylate cyclase (Abeydeera and Day,
1997), which is responsible for the capacitation of swine
spermatozoa. Nonetheless