2.4.1. Effect of incubation period
The influence of different incubation periods (2, 4, 6, 8, 10 and 12 days) on the production of α-amylase was tested. Erlenmeyer flasks (250-ml) containing 50 ml of starch yeast extract liquid medium (pH 6) were autoclaved for 15 min at 1.5 atm and then inoculated with two agar mycelial discs (10 mm diameter) from 7-day-old cultures of the two fungi and incubated at 28°C. Cultures were filtered after 2-day intervals, and the clear supernatants obtained after centrifugation of the filtrates were assayed for amylase activity.