Bacterial strains and materials. All D. radiodurans cultures were
grown at 30 uC in tryptone-yeast extract-glucose (TGY) media (0.5 %
bacto-tryptone, 0.1 % glucose, 0.3 % bacto-yeast extract) with
aeration or on TGY plates supplemented with 1.5 % agar.
Overnight cultures were incubated in fresh TGY medium and the
exponential-phase cells (OD600 0.8) were used for all experiments.
Escherichia coli strains were grown in Luria–Bertani (LB; Colangeli
et al., 2007) broth or on LB plates at 37 uC.