MATERIALS AND METHODS
Spectrophotometer UV Shimadzu, quartz cells, aqueous solutions of doxycycline hyclate in concentrations
100 mg/ml (1); 50 mg/ml (2); 25 mg/ml (3); 12.5 mg/ml (4); 6.25 mg/ml (5) and 3.125 mg/ml (6). A standard
calibration curve of doxycycline was constructed by plotting absorbance versus concentration The dilutions
were analyzed individually in the UV and using their respective absorbance values an absorption curve was
constructed.