Samaranayake et al. (97) evaluated the influence of incubation period, pH and sugar concentration in the culture medium on phospholipase activity. They demonstrated a direct correlation between phospholipase activity and incubation period until the fourth day, when enzymatic activity stabilized. Similarly, phospholipase activity was higher at pH 4.4 than at pH 3.3, but not at pH 5.1 or 6.3, although there was a great fungal growth at higher pH values. There was also a direct correlation between phospholipase activity and galactose or sucrose concentration in the culture medium. Contrastingly, increased glucose concentration (above 100mM) results in the complete cessation of phospholipase activity.