In this study, authors have investigated the ensiling
process as a wet-storage method for the lignocellulosic bioethanol
production in trials performed on maize, rye and
clover grass silages. The effect on the ensiling process was
examined by enzymatic hydrolysis of polysaccharides with
special emphasis on the glucose yield, sugar recovery, and
further conversion to ethanol by the ethanol producing
strains Saccharomyces cerevisiae and Kluyveromyces marxianus.
The reason of using two types of microbes was to observe
differences in yields when ethanol fermentation were run at
two different temperatures, 32 C for S. cerevisiae and 40 C
for K. marxianus, which could influence the ethanol productivity
and also avoid contamination [