3.1. Total phenolics content
All crude extracts from the leaves of neem were used for the
determination of total phenolics content by using Foline
Ciocalteu reagent (Hossain et al., 2013). The results of total
phenolics content by using FolineCiocalteu reagent are presented inTable 1. From Table 1, the highest amount of total
phenolics was obtained in butanol crude extract and the
lowest was chloroform crude extract. The order of total phenolics was butanol>ethyl acetate >water>methanol
>hexane>chloroform. The gallic acid was used for a standard
calibration curve (Table 1andFig. 2).
3.2. Total flavonoids content
Total flavonoid content of all leaves crude extracts of neem
was determined by aluminum chloride method based on
quercetin standard curve (Fig. 3)(Hossain et al., 2013). The
highest amount of total flavonoids was in methanol (529.5 mg/
100 g dry powder samples) and the lowest was in butanol
(63.0 mg//100 g dry powder samples) and followed sequentially methanol (529.5 mg/100 g), hexane (491.0 mg/100 g),
chloroform (400.5 mg/100 g), ethyl acetate (356.5 mg/100 g),
and butanol (63.0 mg/100 g) (Table 2).
3.1. Total phenolics contentAll crude extracts from the leaves of neem were used for thedetermination of total phenolics content by using FolineCiocalteu reagent (Hossain et al., 2013). The results of totalphenolics content by using FolineCiocalteu reagent are presented inTable 1. From Table 1, the highest amount of totalphenolics was obtained in butanol crude extract and thelowest was chloroform crude extract. The order of total phenolics was butanol>ethyl acetate >water>methanol>hexane>chloroform. The gallic acid was used for a standardcalibration curve (Table 1andFig. 2).3.2. Total flavonoids contentTotal flavonoid content of all leaves crude extracts of neemwas determined by aluminum chloride method based onquercetin standard curve (Fig. 3)(Hossain et al., 2013). Thehighest amount of total flavonoids was in methanol (529.5 mg/100 g dry powder samples) and the lowest was in butanol(63.0 mg//100 g dry powder samples) and followed sequentially methanol (529.5 mg/100 g), hexane (491.0 mg/100 g),chloroform (400.5 mg/100 g), ethyl acetate (356.5 mg/100 g),and butanol (63.0 mg/100 g) (Table 2).
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