For Expt. 1, mice were food deprived for 6 h prior to killing after 16 wk of
treatment. Mice were weighed and killed by CO2 overdose. Total percent
body fat was measured postmortem by dual-energy X-ray absorptiometry
using a PIXImus densitometer (Lunar GE Medical Systems). Visceral
adipose tissues (mesenteric, epididymal, and retroperitoneal depots) were
harvested, rinsed, and weighed.