2.4. Lethality concentration determination
The percentage lethality was determined by
comparing the mean surviving larvae of the
test and control tubes. LC50 values were obtained
from the best-fit line plotted concentration
verses percentage lethality. Podophyllotoxin
was used as a positive control in the
bioassay.
2.5. Statistical analysis
The percentage lethality was calculated
from the mean survival larvae of extracts
treated tubes and control. LC50 values were
obtained by best-fit line method.