In order to know if there is any aberration in the regenerated plants, RAPD, a marker that has been revealed to be a potential marker for distinguishing genetic variation (Piccioni et al., 1997; Raimondi et al., 2001), was employed for this purpose, which demonstrated that all of the regenerants showed genetic stability in our regeneration system. Therefore, we can conclude that direct regeneration from leaf did not induce any somaclonal variation that has been depicted in other explants-mediated culture (Cassells and Curry, ).