In the case of feedstock for lipid production, the effluent from the alginate fermentation was recovered, centrifuged for 10 min at 400 g-force, and then passed through a 1.20-µm filter (GF/C, Whatman, UK) to separate the liquid and solid components. The liquid part was then steam autoclaved at 121°C for 20 min.
2.2. Inoculum