3.2.5. Optimization of carbon and nitrogen sources
Completely Randomized Design (CRD) was used to investigate the effect of additional carbon and nitrogen sources as growth supplements. Each of the additional carbon and inexpensive nitrogen supplement was used in different interaction to achieve maximal production of pectin lyase from lemon peel waste. CRD was also applied to study their stimulatory/inhibitory effects under pre-optimized conditions on the production of pectin lyase. The results obtained are summarized in the Table 2. It was recorded that the pectin