Total phenolic and flavonoid content
Total phenolic content was estimated by using Folin-Ciocalteu reagent. Total phenolic content of the different fractions of C. grandis were solvent dependent and expressed as µg pyrocatechol equivalent.. The content of the total phenolics in the fractions decreased in the order of pet-ether > chloroform > ethyl acetate > residual fractions. The total flavonoid content in the fractions was expressed as µg quercetin equivalent. The pet-ether fraction of C. grandis showed highest amount of flavonoids among the fractions tested. The content of total flavonoids in the fractions decreased in the order of pet-ether fraction > residual fraction > ethyl acetate fraction > chloroform fraction (Table 3).