which was
found to be significant (p < 0.01) with a decrease in the optimum temperature of 3◦C per 100 g VS L−1 increase in loading rate. The interaction of pH and loading rate was also found to be near significant (p = 0.06). In general the response surface indicated that higher time (p = 0.03) and loading rates (p < 0.01) could lead to higher concentrations (Fig. 2c). However, when the data for individual trials(>30 g L−1 lactate at 24 h) were evaluated, it was found that at 16 h,