Both nitrogen fixation and Fe‐deficiency stress response are chemically reducing processes. Theoretically either system could enhance the activity of the other. The objective of this study was to determine the interaction of low concentrations of iron and established root nodules on nitrogen fixation in Fe efficient and inefficient soybeans. Nodulated, Fe stressed A7 soybeans (Fe‐efficient) produced an enhanced Fe‐stress response (increased production of H+ ions and reductant at the roots) compared to similarly stressed non‐nodulated plants. The Fe‐inefficient T203 soybean exhibited its standard lack of Fe‐stress response without nodules, but the nodulated T203 actually produced an Fe‐stress response which resulted in less chlorosis.