Strawberries coated silk fibroin retained more water than the no coating controls at day 3 (p < 0.05). An increase in silk fibroin beta-sheets content via water annealing process further decreased fruit dehydration compared to non-water annealed counterparts (p < 0.05) and to the no coating controls (p < 0.05) but no statistically significant difference was found between samples with a beta-sheet content ≥ 36% (p > 0.05).