Based on the flow chart, singleplex PCRs for specific GM events were performed using the published primers (Bonfini, Van den Bulcke, Mazzara, Ben, & Patak, 2012; Kim et al., 2013). These primers were used to confirm specific GM events to be used as positive controls for specific targets. From the 10 samples obtained from South Korean markets, seven samples contained GM soybean. In the 5 samples from Japan, three samples contained detectable levels of the GM soybean event. In the 4 samples from china, one sample contained 3 GM soybean events (RRS, A2704-12, and MON89788). More than 85% of the 4. Conclusion
The developed multiplex PCR system is effective and timesaving for tracking GM soybeans. This screening method is based on 9 screening targets. These targets were successfully tested using all of the available CRMs of GM soybean, which contained the screening elements and 26 soybean-containing food products. The newly developed screening method will cover all potential GM events in soybean that can be present in a sample, as shown in the workflow. This strategy will help monitor and track authorized/unauthorized GM soybean in markets worldwide.
Based on the flow chart, singleplex PCRs for specific GM events were performed using the published primers (Bonfini, Van den Bulcke, Mazzara, Ben, & Patak, 2012; Kim et al., 2013). These primers were used to confirm specific GM events to be used as positive controls for specific targets. From the 10 samples obtained from South Korean markets, seven samples contained GM soybean. In the 5 samples from Japan, three samples contained detectable levels of the GM soybean event. In the 4 samples from china, one sample contained 3 GM soybean events (RRS, A2704-12, and MON89788). More than 85% of the 4. Conclusion The developed multiplex PCR system is effective and timesaving for tracking GM soybeans. This screening method is based on 9 screening targets. These targets were successfully tested using all of the available CRMs of GM soybean, which contained the screening elements and 26 soybean-containing food products. The newly developed screening method will cover all potential GM events in soybean that can be present in a sample, as shown in the workflow. This strategy will help monitor and track authorized/unauthorized GM soybean in markets worldwide.
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