3.1.3. Time course of hydrolysis by cellulase from T. reesei ZU-02
100 g l−1 of cellulosic residue was hydrolyzed by cellulase from T. reesei ZU-02 (20 FPU g−1 substrate; 1.64 CBU g−1 substrate) at pH 4.8 and 50 °C for 60 h. Glucose, xylose, arabinose and cellobiose produced during hydrolysis were analyzed by HPLC at the interval of 12 h (Table 1). The reducing sugar concentration reached 49.4 g l−1 and the hydrolysis yield was 67.5% after 48 h of hydrolysis, and prolonged hydrolysis time beyond 48 h helped little in increasing the hydrolysis yield. Xylose and arabinose were detected out in the hydrolysate, showing the presence of xylanase in T. reesei ZU-02 cellulase.