Aerobic bacteria, coliform, yeast and mould counts in freshly squeezed Chokanan mango juice were 2.74 log CFU/ml,1.00 log CFU/ml, and 2.42 log CFU/ml, respectively. After UV-C treatment and thermal pasteurization, juice samples showed significant reduction of microbial count (p < 0.05), as shown in Table 1. UV-C treatment reduced coliform counts to below detection limits. For aerobic plate count, U60 sample exhibited the highest reduction of microbial load (45%) when compared to other UV-C treated samples, U30 (30%) andU15 (18%). Results showed thermal pasteurization inactivated 100% aerobic bacteria, coliform, yeast and mould in juice sample. High temperature may damage organic molecules (nucleic acidsand proteins) required for the proper functioning of microbial cells, thus causing cellular death.