The Folin-Ciocalteu method (Singleton and Rossi, 1965;Kähkönen et al., 1999) was used to determine the total phenolicscontent. The reaction mixture was prepared by mixing 0.1 mL ofthe extract solution (10 mg/mL), 7.9 mL of distilled water, 0.5 mL ofFolin-Ciocalteu reagent and 1.5 mL of sodium carbonate (20%, w/w).After incubation at room temperature for 1 h, absorbance was mea-sured at 750 nm. The blank was prepared by replacing the extractswith distilled water. Triplicate measurements were made for each