Growth inhibition
R. solani was inoculated onto the center of a plate containing potatoes dextrose agar (PDA) medium, and also performed to NA media, too. After that, four holes were made surround the center by using cork borer, and all of the holes have same distance to the center.
One hundred μl of seven day cultivated endophytic bacterial strain in No.3 medium was put into each hole.
For the negative control, the holes were dropped with distilled water. With the same method was done to F. oxysporum pathogen. Finally, the plates were incubated for 5 days, and growth inhibition area was observed and calculated by the following formula: