were quality assessed with the quality assessment software FastQC [34]. Because Saccharomyces
cerevisiae (S. cerevisiae) is the main food source of lab-reared springtails, we expected that
the sequences could be contaminated with yeast RNA. Also, contamination by human DNA
and by DNA from Wolbachia, an obligate bacterial endosymbiont of F. candida were considered.
To check for potential contamination in both datasets, the raw reads were mapped to the
genomes of Homo sapiens [35], S. cerevisiae [35] and Wolbachia [36] with TopHat 2.0.8 [37]
using default parameters. The raw reads that were not mapped to the above genomes were
used for the assembly.