aerobic bacteria and yeast decreased. Pranoto et
al. (2005) incorporated garlic oil into chitosan film
and the activity of the antimicrobial films was tested
against food pathogens, E. coli, Staphylococcus
aureus, Salmonella typhimurium, L. monocytogenes
and Bacillus cereus. Incorporation of 100 μl of garlic
oil/g of chitosan had antimicrobial activity against
S.aureus, L. monocytogenes, and B. cereus.
The use of orange essential oil in active film
to control mold growth on butter cake is unavailable.
Therefore, this research has 3 main objectives. First,
to test the properties of antimicrobial film from
chitosan incorporating orange essential oil
developed for its use with orange flavored butter
cake. Second, to evaluate the quality and sensory
characteristics of orange flavored butter cake
wrapped by film developed. And third, to test the
inhibitory effect of film developed against mold.