A novel combination between BWand R. oryzae 1526 was applied for the production of FA.
Parameters including fermentation pH, temperature, shaking speed, inoculum size, and total
solid concentration of BW were optimized for higher production of FA. The highest concentration
of FA obtained in this study was 31.3±2.77 g/L. A growth condition of pH 6, 25 °C,
200 rpm, 5 % (v/v) inoculum size, and 25 g/L of TSC supported for the enhanced production of
FA. Spectrophotometric determination of FAwas done in this study. The used substrate served
the purpose of macronutrient and micronutrient for R. oryzae 1526