The increase in 4x:2x nuclei ratios occurred regardless of the type of explant that was treated. As expected, the ratios in control treatments ranged from 0.033 to 0.101, indicating that the majority of nuclei from control leaves were diploid with deviations from 0 (i.e. 100% 2x) caused possibly by sticky nuclei during flow cytometric analysis, endoreduplication, DNA replication
associated with G2 phase of cell division, or spontaneous doubling in vitro