As fermentation proceeded, the amount of proteins extracted in S1 and S2 decreased continually, indicating a progressive decrease in the number of ionic and hydrogen bonds. Most myofibrillar proteins are likely to be solubilised in 0.6 M NaCl under undenatured conditions. Therefore, the fractions solubilised in S1 and S2 could be related to the proteins bound to the network through ionic bonds or hydrogen bonds or to the proteins that were not involved in the formation of the network. Loss in solubility of fermented silver carp mince in S1 and S2 indicated that fish muscle proteins denatured and subsequently aggregated through stronger interactions as pH decreased