2.4. Colony selection
Colonies from strains that grew and produced ethanol at 42 ◦C from (a) and (b)
treatments (IR2-a and IR2-b) were isolated in a maintenance medium, and evaluated
by measuring the CO2 displacement in a growth medium in a standard Eih ¨orn
tube saccharimeter at 40 ◦C and 10 h. The homogeneous cultures that achieved displacement
above 30mm were evaluated measuring the glucose consumption, and
production of biomass and ethanol. The culture with higher ethanol yield (IR2-9a)
and the control (IR2) were tested in SSF.