Four micrometer thick sections were prepared from different
animal groups. Sections were deparaffinised, rehydrated, and endogenous
peroxidase activity was blocked with H2O2 in methanol.
Sections were pre-treated in citrate buffer (pH 6.0) in a microwave.
Sections were incubated at room temperature with monoclonal antiinducible
nitric oxide synthase (iNOS), anti-nuclear factor-κB
(NF-κB), anti-Fas ligand (FasL) and anti-caspase-3 antibodies (Thermo
Scientific, USA, dilution 1:100).