Edible chitosan coatings enriched with essential oil have been shown to have effective antimicrobial activity against some foodborne pathogens. The present work aimed to study the effect of cassava starch, chitosan and Lippia gracilis Schauer genotype (LGRA106 or LGRA107) concentrations on the antimicrobial activity of edible coatings against eight pathogens bacteria using response surface methodology. A total of 17 formulations was prepared according to a central composite design. In LGRA106 and LGRA107, thymol (59.26%) and carvacrol (43.24%) were the major compounds, respectively. Gram-positive bacteria were more susceptible to edible coatings than gram-negative bacteria, more effectively containing LGRA106 than the LGRA107 genotype.